
COVID-19 RT-PCR
CQuentia Lab
The COVID-19 RT-PCR assay in use by CQuentia employs selective amplification of nucleic acid via target-specific primers for ORF 1a, a non-structural region that is unique to SARS-CoV-2, and a conserved region in the structural protein envelope E-gene. This dual target approach allows for both specific detection of SARS-CoV-2, and for pan-Sarbecovirus detection of the sarbecovirus subgenus family that includes SARS-CoV-2.